Oxidation of cysteine 645 of cobalamin-independent methionine synthase causes a methionine limitation in Escherichia coli.
نویسندگان
چکیده
Cobalamin-independent methionine synthase (MetE) catalyzes the final step in Escherichia coli methionine biosynthesis but is inactivated under oxidative conditions, triggering a methionine deficiency. This study demonstrates that the mutation of MetE cysteine 645 to alanine completely eliminates the methionine auxotrophy imposed by diamide treatment, suggesting that modulation of MetE activity via cysteine 645 oxidation has significant physiological consequences for oxidatively stressed cells.
منابع مشابه
Oxidative Stress Inactivates Cobalamin-Independent Methionine Synthase (MetE) in Escherichia coli
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 191 10 شماره
صفحات -
تاریخ انتشار 2009